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Absolute Quantitation of a Heteroplasmic Mitochondrial DNA Deletion Using a Multiplex Three-Primer Real-Time PCR Assay

Quantitation of wild-type and deleted mitochondrial DNA (mtDNA) co-existing within the same cell, (a.k.a. heteroplasmy), is important in mitochondrial disease and aging. We report the development of a multiplex three-primer PCR assay that is capable of absolute quantitation of wild-type and deleted...

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Detaylı Bibliyografya
Asıl Yazarlar: Poe, Bobby G., Navratil, Marian, Arriaga, Edgar A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2006
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1853271/
https://ncbi.nlm.nih.gov/pubmed/17270140
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.12.035
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