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Absolute Quantitation of a Heteroplasmic Mitochondrial DNA Deletion Using a Multiplex Three-Primer Real-Time PCR Assay
Quantitation of wild-type and deleted mitochondrial DNA (mtDNA) co-existing within the same cell, (a.k.a. heteroplasmy), is important in mitochondrial disease and aging. We report the development of a multiplex three-primer PCR assay that is capable of absolute quantitation of wild-type and deleted...
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| Autors principals: | , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2006
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1853271/ https://ncbi.nlm.nih.gov/pubmed/17270140 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.12.035 |
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