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DNA supercoiling suppresses real-time PCR: a new approach to the quantification of mitochondrial DNA damage and repair
As a gold standard for quantification of starting amounts of nucleic acids, real-time PCR is increasingly used in quantitative analysis of mtDNA copy number in medical research. Using supercoiled plasmid DNA and mtDNA modified both in vitro and in cancer cells, we demonstrated that conformational ch...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2007
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1851651/ https://ncbi.nlm.nih.gov/pubmed/17284464 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm010 |
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