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Cloning of a lactate dehydrogenase gene from Clostridium acetobutylicum B643 and expression in Escherichia coli.

A lactate dehydrogenase (LDH) gene of Clostridium acetobutylicum B643 was cloned on two recombinant plasmids, pPC37 and pPC58, that were selected by complementation of Escherichia coli PRC436 (acd), a fermentation-defective mutant that does not grow anaerobically on glucose. E. coli PRC436(pPC37) an...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Contag, P R, Williams, M G, Rogers, P
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1990
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC185064/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2082823/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.12.3760-3765.1990
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