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Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: Studies in vitro, in animals, and in humans

A method for measuring DNA synthesis and, thus, cell proliferation, in vivo is presented. The technique consists of administering [6,6-(2)H(2)]Glc or [U-(13)C]Glc, isolating genomic DNA, hydrolyzing enzymatically to free deoxyribonucleosides, and derivatizing for GC-MS analysis of dA or dG isotopic...

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Autori principali: Macallan, Derek C., Fullerton, Catherine A., Neese, Richard A., Haddock, Katherine, Park, Sunny S., Hellerstein, Marc K.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC18485/
https://ncbi.nlm.nih.gov/pubmed/9435257
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