Stable Isotope Labeling with Amino acids in Nematodes

We describe an approach for the accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) for nematodes, by feeding worms a heavy lysine- and arginine-labeled E. coli strain. We also report a genetic soluti...

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Main Authors: Larance, Mark, Bailly, Aymeric P., Pourkarimi, Ehsan, Hay, Ronald T., Buchanan, Grant, Coulthurst, Sarah, Xirodimas, Dimitris P., Gartner, Anton, Lamond, Angus I.
格式: Artigo
語言:Inglês
出版: 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3184259/
https://ncbi.nlm.nih.gov/pubmed/21874007
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1679
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