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Stable Isotope Labeling with Amino acids in Nematodes
We describe an approach for the accurate quantitation of global protein dynamics in Caenorhabditis elegans. We adapted Stable Isotope Labeling with Amino acids in Cell culture (SILAC) for nematodes, by feeding worms a heavy lysine- and arginine-labeled E. coli strain. We also report a genetic soluti...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3184259/ https://ncbi.nlm.nih.gov/pubmed/21874007 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1679 |
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