Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates

Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immo...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Nieves, Rafael A., Ellis, Robert P., Todd, Roberta J., Johnson, Timothy J. A., Grohmann, Karel, Himmel, Michael E.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1991
Soggetti:	Physiology and Biotechnology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183942/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348581/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.11.3163-3170.1991
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