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COMPARISON OF THE EXPRESSION IN Saccharomyces cerevisiae OF ENDOGLUCANASE II FROM Trichoderma reesei AND ENDOGLUCANASE I FROM Aspergillus aculeatus

Two distinct expression cassettes were synthesized by overlapping PCR for expressing the endoglucanase I gene (egl1) from Aspergillus aculeatus and the endoglucanase II gene (egl2) from Trichoderma reesei in a Saccharomyces cerevisiae host. One contained the anchored sequence from the S. cerevisiae...

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Principais autores: Weina Zhang, Cheng Liu, Guoqiang Wang, Yuanyuan Ma, Kun Zhang, Shaolan Zou, Minhua Zhang
Formato: Artigo
Idioma:Inglês
Publicado em: North Carolina State University 2012-07-01
coleção:BioResources
Assuntos:
Endoglucanase
Aspergillus aculeatus
Trichoderma reesei
Expression in yeast
Enzymatic activity
Viscosity
Acesso em linha:http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_07_3_4031_Zhang_Expression_Saccharomyces_Endoglucanase_Trichoderma_Aspergillus/1656
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