Visualization of Trichoderma reesei Cellobiohydrolase I and Endoglucanase I on Aspen Cellulose by Using Monoclonal Antibody-Colloidal Gold Conjugates

Monoclonal antibodies (MAbs) specific for cellobiohydrolase I (CBH I) and endoglucanase I (EG I) were conjugated to 10- and 15-nm colloidal gold particles, respectively. The binding of CBH I and EG I was visualized by utilizing the MAb-colloidal gold probes. The visualization procedure involved immo...

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Bibliographic Details
Main Authors: Nieves, Rafael A., Ellis, Robert P., Todd, Roberta J., Johnson, Timothy J. A., Grohmann, Karel, Himmel, Michael E.
Format: Artigo
Language:Inglês
Published: 1991
Subjects:
Physiology and Biotechnology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC183942/
https://ncbi.nlm.nih.gov/pubmed/16348581
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