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Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.

Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspensi...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Pillai, S D, Josephson, K L, Bailey, R L, Gerba, C P, Pepper, I L
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1991
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183564/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1662931/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.8.2283-2286.1991
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