Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.

Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspensi...

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Dettagli Bibliografici

Pubblicato in:	Appl Environ Microbiol
Autori principali:	Pillai, S D, Josephson, K L, Bailey, R L, Gerba, C P, Pepper, I L
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183564/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1662931/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.8.2283-2286.1991
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