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Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.

Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspensi...

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Detalhes bibliográficos
Main Authors: Pillai, S D, Josephson, K L, Bailey, R L, Gerba, C P, Pepper, I L
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC183564/
https://ncbi.nlm.nih.gov/pubmed/1662931
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