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Rapid method for processing soil samples for polymerase chain reaction amplification of specific gene sequences.

Bacterial cells can be differentially separated from soil colloids on the basis of their buoyant densities. By using this principle, a modified sucrose gradient centrifugation protocol has been developed for separating bacterial cells from most of the soil colloids. Since the bacterial cell suspensi...

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Bibliografische gegevens
Gepubliceerd in:Appl Environ Microbiol
Hoofdauteurs: Pillai, S D, Josephson, K L, Bailey, R L, Gerba, C P, Pepper, I L
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology (ASM) 1991
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183564/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1662931/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.8.2283-2286.1991
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