Successful approach for detection of low numbers of enterotoxigenic Escherichia coli in minced meat by using the polymerase chain reaction.

The polymerase chain reaction (PCR) was used as a tool for the detection of enterotoxigenic Escherichia coli in minced meat. With two synthetic 29-mer oligonucleotides, a 195-bp fragment from the E. coli heat-labile enterotoxin (LT) gene could be amplified specifically. When 6 CFU was added to the r...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Wernars, K, Delfgou, E, Soentoro, P S, Notermans, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183499/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1892381/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.7.1914-1919.1991
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