Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis

New rpoB gene primers for detecting Rif(r) in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rif(r) isolates examined, 97% had mutations within cluster I,...

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Hlavní autoři: Rigouts, Leen, Nolasco, Oscar, de Rijk, Pim, Nduwamahoro, Elie, Van Deun, Armand, Ramsay, Andy, Arevalo, Jorge, Portaels, Françoise
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2007
Témata:
Mycobacteriology and Aerobic Actinomycetes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828994/
https://ncbi.nlm.nih.gov/pubmed/17093024
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01489-06
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