Cloning of the structural gene for Clostridium botulinum type C1 toxin and whole nucleotide sequence of its light chain component.

The toxigenicity of Clostridium botulinum type C1 is mediated by specific bacteriophages. DNA was extracted from one of these phages. Two DNA fragments, 3 and 7.8 kb, which produced the protein reacting with antitoxin serum were cloned by using bacteriophage lambda gt11 and Escherichia coli. Both DN...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Kimura, K, Fujii, N, Tsuzuki, K, Murakami, T, Indoh, T, Yokosawa, N, Oguma, K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182863/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2059039/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.4.1168-1172.1991
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