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Cloning of the structural gene for Clostridium botulinum type C1 toxin and whole nucleotide sequence of its light chain component.

The toxigenicity of Clostridium botulinum type C1 is mediated by specific bacteriophages. DNA was extracted from one of these phages. Two DNA fragments, 3 and 7.8 kb, which produced the protein reacting with antitoxin serum were cloned by using bacteriophage lambda gt11 and Escherichia coli. Both DN...

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Main Authors: Kimura, K, Fujii, N, Tsuzuki, K, Murakami, T, Indoh, T, Yokosawa, N, Oguma, K
Format: Artigo
Jezik:Inglês
Izdano: 1991
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC182863/
https://ncbi.nlm.nih.gov/pubmed/2059039
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