Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus.

A genomic DNA fragment encoding aminoacylase activity of the eubacterium Bacillus stearothermophilus was cloned into Escherichia coli. Transformants expressing aminoacylase activity were selected by their ability to complement E. coli mutants defective in acetylornithine deacetylase activity, the en...

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Detalhes bibliográficos
Main Authors: Sakanyan, V, Desmarez, L, Legrain, C, Charlier, D, Mett, I, Kochikyan, A, Savchenko, A, Boyen, A, Falmagne, P, Pierard, A
Formato: Artigo
Idioma:Inglês
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC182544/
https://ncbi.nlm.nih.gov/pubmed/8285691
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