Gene cloning, sequence analysis, purification, and characterization of a thermostable aminoacylase from Bacillus stearothermophilus.

A genomic DNA fragment encoding aminoacylase activity of the eubacterium Bacillus stearothermophilus was cloned into Escherichia coli. Transformants expressing aminoacylase activity were selected by their ability to complement E. coli mutants defective in acetylornithine deacetylase activity, the en...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Sakanyan, V, Desmarez, L, Legrain, C, Charlier, D, Mett, I, Kochikyan, A, Savchenko, A, Boyen, A, Falmagne, P, Pierard, A
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182544/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8285691/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.11.3878-3888.1993
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