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Rapid Mini-Prep Isolation of High-Quality Plasmid DNA from Lactococcus and Lactobacillus spp.

A simple, rapid plasmid mini-prep procedure for lactococci and lactobacilli which gives high yields and can be performed on overnight broth cultures is presented. Large plasmids were isolated from both lactococci and lactobacilli, including a 70-kb plasmid from Lactobacillus acidophilus C7. The puri...

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Bibliographic Details
Main Authors: O'Sullivan, Daniel J., Klaenhammer, Todd R.
Format: Artigo
Language:Inglês
Published: 1993
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC182348/
https://ncbi.nlm.nih.gov/pubmed/16349028
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