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Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy

The brightness of fluorescence fluctuations provides information about protein interactions in the intercellular environment under equilibrium conditions. Here we demonstrate that the stoichiometry of a protein complex containing two proteins labeled with CFP and YFP can be determined by brightness...

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Autors principals: Chen, Yan, Müller, Joachim D.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1805531/
https://ncbi.nlm.nih.gov/pubmed/17307882
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0606557104
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