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Determining the stoichiometry of protein heterocomplexes in living cells with fluorescence fluctuation spectroscopy
The brightness of fluorescence fluctuations provides information about protein interactions in the intercellular environment under equilibrium conditions. Here we demonstrate that the stoichiometry of a protein complex containing two proteins labeled with CFP and YFP can be determined by brightness...
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| Autores principales: | , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
2007
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1805531/ https://ncbi.nlm.nih.gov/pubmed/17307882 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0606557104 |
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