Rapid Separation and Concentration of Food-Borne Pathogens in Food Samples Prior to Quantification by Viable-Cell Counting and Real-Time PCR

Buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as PCR, and to prevent false-positive results due to DNA originating from dead cells. Applying a principle of buoyant densi...

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Bibliographic Details
Main Authors: Fukushima, Hiroshi, Katsube, Kazunori, Hata, Yukiko, Kishi, Ryoko, Fujiwara, Satomi
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2007
Subjects:
Food Microbiology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1797114/
https://ncbi.nlm.nih.gov/pubmed/17056684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01772-06
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