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Rapid Separation and Concentration of Food-Borne Pathogens in Food Samples Prior to Quantification by Viable-Cell Counting and Real-Time PCR

Buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as PCR, and to prevent false-positive results due to DNA originating from dead cells. Applying a principle of buoyant densi...

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Autori principali: Fukushima, Hiroshi, Katsube, Kazunori, Hata, Yukiko, Kishi, Ryoko, Fujiwara, Satomi
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1797114/
https://ncbi.nlm.nih.gov/pubmed/17056684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01772-06
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