Rapid Separation and Concentration of Food-Borne Pathogens in Food Samples Prior to Quantification by Viable-Cell Counting and Real-Time PCR

Buoyant density gradient centrifugation has been used to separate bacteria from complex food matrices, as well as to remove compounds that inhibit rapid detection methods, such as PCR, and to prevent false-positive results due to DNA originating from dead cells. Applying a principle of buoyant densi...

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Bibliografiska uppgifter
Huvudupphovsmän: Fukushima, Hiroshi, Katsube, Kazunori, Hata, Yukiko, Kishi, Ryoko, Fujiwara, Satomi
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology 2007
Ämnen:
Food Microbiology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1797114/
https://ncbi.nlm.nih.gov/pubmed/17056684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01772-06
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