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Reversible Unfolding of the Severe Acute Respiratory Syndrome Coronavirus Main Protease in Guanidinium Chloride

Chemical denaturant sensitivity of the dimeric main protease from severe acute respiratory syndrome (SARS) coronavirus to guanidinium chloride was examined in terms of fluorescence spectroscopy, circular dichroism, analytical ultracentrifuge, and enzyme activity change. The dimeric enzyme dissociate...

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Detalhes bibliográficos
Main Authors: Chang, Hui-Ping, Chou, Chi-Yuan, Chang, Gu-Gang
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1783898/
https://ncbi.nlm.nih.gov/pubmed/17142288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.091736
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