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Mechanism for Intein C-Terminal Cleavage: A Proposal from Quantum Mechanical Calculations

Inteins are autocatalytic protein cleavage and splicing elements. A cysteine to alanine mutation at the N-terminal of inteins inhibits splicing and isolates the C-terminal cleavage reaction. Experiments indicate an enhanced C-terminal cleavage reaction rate upon decreasing the solution pH for the cl...

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Bibliografski detalji
Glavni autori: Shemella, Philip, Pereira, Brian, Zhang, Yiming, Van Roey, Patrick, Belfort, Georges, Garde, Shekhar, Nayak, Saroj K.
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2007
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1779973/
https://ncbi.nlm.nih.gov/pubmed/17085503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.092049
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