A conserved threonine spring-loads precursor for intein splicing

Protein splicing is an autocatalytic process where an “intein” self-cleaves from a precursor and ligates the flanking N- and C-“extein” polypeptides. Inteins occur in all domains of life and have myriad uses in biotechnology. Although the reaction steps of protein splicing are known, mechanistic det...

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Bibliographic Details
Main Authors: Dearden, Albert K, Callahan, Brian, Roey, Patrick Van, Li, Zhong, Kumar, Utsav, Belfort, Marlene, Nayak, Saroj K
Format: Artigo
Language:Inglês
Published: Wiley Subscription Services, Inc., A Wiley Company 2013
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3649257/
https://ncbi.nlm.nih.gov/pubmed/23423655
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2236
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