Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus

We constructed a “temperature-jump/stopped-flow” apparatus that allows us to study fast enzyme reactions at extremely high temperatures. This apparatus is a redesigned stopped-flow which is capable of mixing the reactants on a submillisecond timescale concomitant with a temperature-jump even as larg...

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Autors principals: Kintses, Bálint, Simon, Zoltán, Gyimesi, Máté, Tóth, Júlia, Jelinek, Balázs, Niedetzky, Csaba, Kovács, Mihály, Málnási-Csizmadia, András
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2006
Matèries:
Spectroscopy, Imaging, Other Techniques
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1779911/
https://ncbi.nlm.nih.gov/pubmed/17012324
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.092833
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