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Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus
We constructed a “temperature-jump/stopped-flow” apparatus that allows us to study fast enzyme reactions at extremely high temperatures. This apparatus is a redesigned stopped-flow which is capable of mixing the reactants on a submillisecond timescale concomitant with a temperature-jump even as larg...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1779911/ https://ncbi.nlm.nih.gov/pubmed/17012324 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.092833 |
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