Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.

Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycoba...

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Bibliografische gegevens

Gepubliceerd in:	J Bacteriol
Hoofdauteurs:	Pelicic, V, Reyrat, J M, Gicquel, B
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1996
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8576057/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.4.1197-1199.1996
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