Expression of the Bacillus subtilis sacB gene confers sucrose sensitivity on mycobacteria.

Expression in mycobacteria of the structural gene sacB, which encodes the Bacillus subtilis levansucrase, was investigated. sacB expression is lethal to Mycobacterium smegmatis and Mycobacterium bovis BCG in the presence of 10% sucrose. sacB could thus be used as a counterselectable marker in mycoba...

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Pubblicato in:	J Bacteriol
Autori principali:	Pelicic, V, Reyrat, J M, Gicquel, B
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1996
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177784/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8576057/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.178.4.1197-1199.1996
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