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Isolation and Characterization of Ribosomes and Translation Initiation Factors from the Gram-Positive Soil Bacterium Streptomyces lividans
A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderles...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2004
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC522217/ https://ncbi.nlm.nih.gov/pubmed/15466040 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.186.20.6864-6875.2004 |
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