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Markerless deletions of pil genes in Myxococcus xanthus generated by counterselection with the Bacillus subtilis sacB gene.

In-frame deletions of pilA and pilS were constructed in Myxococcus xanthus with a plasmid integration-excision strategy facilitated by sacB. sacB conferred sucrose sensitivity upon its M. xanthus host only when it lay in the same orientation as adjacent M. xanthus genes. Gene orientation also affect...

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Библиографические подробности
Главные авторы: Wu, S S, Kaiser, D
Формат: Artigo
Язык:Inglês
Опубликовано: 1996
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC178429/
https://ncbi.nlm.nih.gov/pubmed/8824635
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