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Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.

Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti ex...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Rushing, B G, Long, S R
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1995
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177565/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7592490/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.23.6952-6957.1995
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