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Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.
Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti ex...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1995
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC177565/ https://ncbi.nlm.nih.gov/pubmed/7592490 |
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