Cloning and characterization of the sigA gene encoding the major sigma subunit of Rhizobium meliloti.
Using PCR to create a probe based on conserved region 2 of sigma factors, we have cloned the sigA gene coding for the major sigma factor of Rhizobium meliloti. The 684-residue protein encoded by the sigA gene was expressed in vitro in coupled transcription-translation experiments with R. meliloti ex...
Shranjeno v:
| izdano v: | J Bacteriol |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1995
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC177565/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7592490/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.23.6952-6957.1995 |
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