Using SEC-RPLC and RPLC-CIEF as two-dimensional separation strategies for protein profiling

Bottom-up proteomics (analyzing peptides that result from protein digestion) has demonstrated capability for broad proteome coverage and good throughput. However, due to incomplete sequence coverage, this approach is not ideally suited to the study of modified proteins. The modification complement o...

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Bibliographische Detailangaben
Hauptverfasser: Simpson, David C., Ahn, Seonghee, Pasa-Tolic, Ljiljana, Bogdanov, Bogdan, Mottaz, Heather M., Vilkov, Andrey N., Anderson, Gordon A., Lipton, Mary S., Smith, Richard D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2006
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1769308/
https://ncbi.nlm.nih.gov/pubmed/16732621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.200600037
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