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Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase

We used engineered zinc finger peptides (ZFPs) to bind selectively to predetermined sequences in human mtDNA. Surprisingly, we found that engineered ZFPs cannot be reliably routed to mitochondria by using only conventional mitochondrial targeting sequences. We here show that addition of a nuclear ex...

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Autori principali: Minczuk, Michal, Papworth, Monika A., Kolasinska, Paulina, Murphy, Michael P., Klug, Aaron
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2006
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1750892/
https://ncbi.nlm.nih.gov/pubmed/17170133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0609502103
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