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Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase

We used engineered zinc finger peptides (ZFPs) to bind selectively to predetermined sequences in human mtDNA. Surprisingly, we found that engineered ZFPs cannot be reliably routed to mitochondria by using only conventional mitochondrial targeting sequences. We here show that addition of a nuclear ex...

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Bibliografiske detaljer
Main Authors: Minczuk, Michal, Papworth, Monika A., Kolasinska, Paulina, Murphy, Michael P., Klug, Aaron
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2006
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1750892/
https://ncbi.nlm.nih.gov/pubmed/17170133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0609502103
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