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Sequence-specific modification of mitochondrial DNA using a chimeric zinc finger methylase

We used engineered zinc finger peptides (ZFPs) to bind selectively to predetermined sequences in human mtDNA. Surprisingly, we found that engineered ZFPs cannot be reliably routed to mitochondria by using only conventional mitochondrial targeting sequences. We here show that addition of a nuclear ex...

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Hlavní autoři: Minczuk, Michal, Papworth, Monika A., Kolasinska, Paulina, Murphy, Michael P., Klug, Aaron
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1750892/
https://ncbi.nlm.nih.gov/pubmed/17170133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0609502103
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