Deletion of purE attenuates Brucella melitensis 16M for growth in human monocyte-derived macrophages.

We constructed a defined purine-auxotrophic mutant of Brucella melitensis 16M by chromosomal gene replacement. We electroporated B. melitensis 16M with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of purE, the intergenic region, and 18 bases of...

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Hlavní autoři: Drazek, E S, Houng, H S, Crawford, R M, Hadfield, T L, Hoover, D L, Warren, R L
Médium: Artigo
Jazyk:Inglês
Vydáno: 1995
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Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC173454/
https://ncbi.nlm.nih.gov/pubmed/7642258
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