Deletion of purE attenuates Brucella melitensis 16M for growth in human monocyte-derived macrophages.

We constructed a defined purine-auxotrophic mutant of Brucella melitensis 16M by chromosomal gene replacement. We electroporated B. melitensis 16M with suicide plasmids containing a kanamycin resistance cassette that replaced 226 bp at the carboxyl end of purE, the intergenic region, and 18 bases of...

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Detalhes bibliográficos

Publicado no:	Infect Immun
Principais autores:	Drazek, E S, Houng, H S, Crawford, R M, Hadfield, T L, Hoover, D L, Warren, R L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC173454/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7642258/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.63.9.3297-3301.1995
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