Purification of Staphylococcus aureus beta-lactamases by using sequential cation-exchange and affinity chromatography.
Boronic acids are active-site inhibitors of serine beta-lactamases, and a phenylboronic acid-agarose affinity column has been used to purify beta-lactamase from crude cell extracts of several bacterial species. We applied phenylboronic acid-agarose chromatography to the purification of Staphylococcu...
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| Publicado no: | Antimicrob Agents Chemother |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172020/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2073108/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.34.11.2177 |
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