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Purification of Staphylococcus aureus beta-lactamases by using sequential cation-exchange and affinity chromatography.

Boronic acids are active-site inhibitors of serine beta-lactamases, and a phenylboronic acid-agarose affinity column has been used to purify beta-lactamase from crude cell extracts of several bacterial species. We applied phenylboronic acid-agarose chromatography to the purification of Staphylococcu...

詳細記述

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書誌詳細
主要な著者: Kernodle, D S, Zygmunt, D J, McGraw, P A, Chipley, J R
フォーマット: Artigo
言語:Inglês
出版事項: 1990
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC172020/
https://ncbi.nlm.nih.gov/pubmed/2073108
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