Purification of Staphylococcus aureus beta-lactamases by using sequential cation-exchange and affinity chromatography.

Boronic acids are active-site inhibitors of serine beta-lactamases, and a phenylboronic acid-agarose affinity column has been used to purify beta-lactamase from crude cell extracts of several bacterial species. We applied phenylboronic acid-agarose chromatography to the purification of Staphylococcu...

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Veröffentlicht in:	Antimicrob Agents Chemother
Hauptverfasser:	Kernodle, D S, Zygmunt, D J, McGraw, P A, Chipley, J R
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC172020/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2073108/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aac.34.11.2177
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