Purification of Staphylococcus aureus beta-lactamases by using sequential cation-exchange and affinity chromatography.

Boronic acids are active-site inhibitors of serine beta-lactamases, and a phenylboronic acid-agarose affinity column has been used to purify beta-lactamase from crude cell extracts of several bacterial species. We applied phenylboronic acid-agarose chromatography to the purification of Staphylococcu...

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Main Authors: Kernodle, D S, Zygmunt, D J, McGraw, P A, Chipley, J R
Format: Artigo
Jezik:Inglês
Izdano: 1990
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC172020/
https://ncbi.nlm.nih.gov/pubmed/2073108
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