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Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli
The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whe...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Springer-Verlag
2006
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1705536/ https://ncbi.nlm.nih.gov/pubmed/16770590 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00449-006-0069-9 |
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