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Production of a recombinant polyester-cleaving hydrolase from Thermobifida fusca in Escherichia coli

The hydrolase (Thermobifida fusca hydrolase; TfH) from T. fusca was produced in Escherichia coli as fusion protein using the OmpA leader sequence and a His(6) tag. Productivity could be raised more than 100-fold. Both batch and fed-batch cultivations yield comparable cell specific productivities whe...

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Hlavní autoři: Dresler, Karolin, van den Heuvel, Joop, Müller, Rolf-Joachim, Deckwer, Wolf-Dieter
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer-Verlag 2006
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1705536/
https://ncbi.nlm.nih.gov/pubmed/16770590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00449-006-0069-9
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