Regionalized GC content of template DNA as a predictor of PCR success
A set of 1438 human exons was subjected to nested PCR. The initial success rate using a standard PCR protocol required for ligation-independent cloning was 83.4%. Logistic regression analysis was conducted on 27 primer- and template-related characteristics, of which most could be ignored apart from...
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| Опубліковано в:: | Nucleic Acids Res |
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| Автори: | , , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Oxford University Press
2003
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169991/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907751/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng101 |
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