Regionalized GC content of template DNA as a predictor of PCR success

A set of 1438 human exons was subjected to nested PCR. The initial success rate using a standard PCR protocol required for ligation-independent cloning was 83.4%. Logistic regression analysis was conducted on 27 primer- and template-related characteristics, of which most could be ignored apart from...

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izdano v:	Nucleic Acids Res
Principais autores:	Benita, Yair, Oosting, Ronald S., Lok, Martin C., Wise, Michael J., Humphery-Smith, Ian
Format:	Artigo
Jezik:	Inglês
Izdano:	Oxford University Press 2003
Teme:	NAR Methods Online
Online dostop:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169991/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12907751/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng101
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