General plasmids for producing RNA in vitro transcripts with homogeneous ends
In vitro transcripts of bacteriophage RNA polymerases (RNAPs), such as T7 RNAP, often suffer from a considerable degree of 3′-end heterogeneity and, with certain promoter sequences, 5′-end heterogeneity. For some applications, this transcript heterogeneity poses a significant problem. A potential so...
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| Publicado no: | Nucleic Acids Res |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169970/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888534/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng082 |
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