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General plasmids for producing RNA in vitro transcripts with homogeneous ends

In vitro transcripts of bacteriophage RNA polymerases (RNAPs), such as T7 RNAP, often suffer from a considerable degree of 3′-end heterogeneity and, with certain promoter sequences, 5′-end heterogeneity. For some applications, this transcript heterogeneity poses a significant problem. A potential so...

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Autors principals: Walker, Scott C., Avis, Johanna M., Conn, Graeme L.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2003
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC169970/
https://ncbi.nlm.nih.gov/pubmed/12888534
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