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General plasmids for producing RNA in vitro transcripts with homogeneous ends
In vitro transcripts of bacteriophage RNA polymerases (RNAPs), such as T7 RNAP, often suffer from a considerable degree of 3′-end heterogeneity and, with certain promoter sequences, 5′-end heterogeneity. For some applications, this transcript heterogeneity poses a significant problem. A potential so...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2003
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC169970/ https://ncbi.nlm.nih.gov/pubmed/12888534 |
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