General plasmids for producing RNA in vitro transcripts with homogeneous ends

In vitro transcripts of bacteriophage RNA polymerases (RNAPs), such as T7 RNAP, often suffer from a considerable degree of 3′-end heterogeneity and, with certain promoter sequences, 5′-end heterogeneity. For some applications, this transcript heterogeneity poses a significant problem. A potential so...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Walker, Scott C., Avis, Johanna M., Conn, Graeme L.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2003
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169970/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888534/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng082
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