Fast and accurate determination of sites along the FUT2 in vitro transcript that are accessible to antisense oligonucleotides by application of secondary structure predictions and RNase H in combination with MALDI-TOF mass spectrometry

Alteration of gene expression by use of antisense oligonucleotides has considerable potential for therapeutic purposes and scientific studies. Although applied for almost 25 years, this technique is still associated with difficulties in finding antisense-effective regions along the target mRNA. This...

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Εκδόθηκε σε:	Nucleic Acids Res
Κύριοι συγγραφείς:	Gabler, Angelika, Krebs, Stefan, Seichter, Doris, Förster, Martin
Μορφή:	Artigo
Γλώσσα:	Inglês
Έκδοση:	Oxford University Press 2003
Θέματα:	NAR Methods Online
Διαθέσιμο Online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC169965/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12888531/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng079
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